A new antibiotic named 61-26 active against gram-positive bacteria and some fungi was isolated from a Bacillus strain. The antibiotic is a weakly basic peptide slightly soluble in aqueous alcohols. An approximate empirical formula of C50H93-N11O17 and constituent amino acids of aspartic acid (1 mole), serine (2 moles), alanine (2 moles), and sum of valine and isoleucine (2 moles) are indicated.
