MUTATIONAL BIOSYNTHESIS OF BUTIROSIN ANALOGS
I. CONVERSION OF NEAMINE ANALOGS INTO BUTIROSIN ANALOGS BY MUTANTS OF BACILLUS CIRCULANS
1978 年 31 巻 10 号 p. 1023-1030
詳細
1978 年 31 巻 10 号 p. 1023-1030
By N-methyl-N'-nitro-N-nitrosoguanidine treatment, neamine-negative mutants which required neamine for biosynthesis of butirosins were obtained from a butirosin-producing organism Bacillus circulars. These mutants also produced butirosins from paromamine and could be divided into two types I and II. Mutants of type I could not produce butirosins from 2-deoxystreptamine, whereas those of type II could. Two typical mutants MCRL 5003 (type I) and MCRL 5004 (type II) could produce butirosin analogs, 3', 4'-dideoxybutirosins, 6'-N-methylbutirosins, 3', 4'-dideoxy-6'-N-methylbutirosins and 3', 4'-dideoxy-6'-C-methylbutirosins from neamine analogs, gentamine C1a, 6'-N-methylneamine, 6'-N-methylgentamine C1a, and gentamine C2, respectively.
