DEACETYLATION OF PS-5, A NEW β-LACTAM COMPOUND

II. SEPARATION AND PURIFICATION OF L- AND D-AMINO ACID ACYLASES FROM PSEUDOMONAS SP. 1158

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L-Amino acid acylase and D-amino acid acylase of Pseudomonas sp . 1158 which converted PS-5 to NS-5 (deacetylated PS-5) were separated and purified by sonication, streptomycin and Ammonium sulfate fractionations, DEAE-Sephacel column chromatography and gel filtration. Molecular weight and the isoelectric point were estimated to be 75, 000 and pI 5.45 for L-amino acid acylase and 100, 000 and pI 4.95 for D-amino acid acylase.