ISOLATION AND STRUCTURE DETERMINATION OF CRISAMICIN A, A NEW ANTIBIOTIC FROM MICROMONOSPORA PURPUREOCHROMOGENES SUBSP. HALOTOLERANS

Abstract

A new antibiotic, crisamicin A, with in vitro activity against Gram-positive bacteria, B16 murine melanoma cells, and herpes simplex, vaccinia, and vesicular stomatitis viruses, has been isolated from Micromonospora purpureochromogenes subsp. halotolerans. On the basis of ¹H and ¹³C NMR spectroscopic, high resolution field desorption mass spectrometric, and circular dichroism studies of the antibiotic and several of its derivatives, the structure of crisamicin A has been assigned.