BIOCHEMICAL STUDIES ON 2-DEOXY-SCYLLO-INOSOSE, AN EARLY INTERMEDIATE IN THE BIOSYNTHESIS OF 2-DEOXYSTREPTAMINE
II. QUANTITATIVE ANALYSIS OF 2-DEOXY-SCYLLO-INOSOSE
1992 年 45 巻 5 号 p. 767-773
詳細
1992 年 45 巻 5 号 p. 767-773
A practical quantitative analysis of 2-deoxy-scyllo-inosose by means of GC-MS selected-ion monitoring (SIM) was exploited in order to assay the enzymatic 2-deoxy-scyllo-inosose formation, the first stage of the 2-deoxystreptamine biosynthesis. Mass spectral fragmentations of 2-deoxy-scyllo-inosose tetra-O-trimethylsilyl (TMS) ether was also investigated by the use of [2, 2-2H2]-2-deoxy-scyllo-inosose.
