The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
Induction of Differentiation of HL-60 Cells by the Anti-fungal Antibiotic, Radicicol
YOKO SHIMADATOMOKO OGAWAAIYA SATOISAO KANEKOYOSHIO TSUJITA
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1995 Volume 48 Issue 8 Pages 824-830

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Abstract

The anti-fungal antibiotic, radicicol, produced in the culture broth of Neocosmospora tenuicristata, was found to induce differentiation of HL-60 cells into macrophages from the following evidence: (1) it caused morphological changes into macrophage-like cells, (2) induced NBT (Nitrobluetetrazolium) reduction activity, (3) induced phagocytosis, and (4) induced α-naphthyl acetate esterase activity. The concentration of radicicol required to differentiate HL-60 cells is 50-100ng/ml, and the incubation time required for commitment of differentiation is 16 hours. Flow cytometry analysis indicated that radicicol blocks the cell cycle of HL-60 cells at the Gl and G2 sites. In addition, radicicol induced reversal of the transformed phenotype of ras-transformed NIH3T3 cells (DT cells) at 25ng/ml.

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