Abstract
Pharmacological effects of DKB, a new aminoglycoside antibiotic, were studied and following results were obtained.
1) In urethane (1g/kg, s.c.) anesthetized rabbit, administration of 10mg/kg (i. v.) of DKB caused a fall of blood pressure and a slight acceleration of respiration. Administration of 20mg/kg of DKB caused decrease in heart rate on ECG. These cardiovascular effects of DKB were retained following the administration of atropine, diphenhydramine, propranolol and sodium nitrite, and after vagotomy and spinal resection. On the isolated rabbit ear preparation, blood vessels were slightly dilated following the administration of 10% DKB.
2) The movements of isolated rabbit intestine and rat uterus in MAGNUS apparatus were inhibited by the administration of 0.005% DKB. The tonus of isolated guinea-pig trachea strip chain in MAGNUS apparatus was relaxed by the administration of 0.01% DKB. These effects of DKB were antagonized by the addition of BaCl2 and acetylcholine.
3) The responses of the phrenic nerve-diaphragm preparation of rat to nerve stimulation were blocked by the administration of 0.3% DKB. This effect of DKB was antagonized by the addition of CaCl2 and pentobarbital.
4) At high concentrated of DKB, irritation to the muscle of rat was observed, and permeability of skin vessels were stimulated by the administration of 10% DKB.
5) No significant effects were observed on central nervous system, body temperature, red cell resistance and prothrombin time by the administration of DKB.
6) No antigenicity in rabbit was formed by the administration of DKB. At the therapeutic doses of DKB, no remarkable pharmacological actions were observed.
