ANTHRACYCLINE ANTIBIOTIC 2-HYDROXYACLACINOMYCINS

I. 2-HYDROXYACLACINOMYCIN-PRODUCING RECOMBINANT OBTAINED FROM ACLACINOMYCIN-BLOCKED MUTANTS OF STREPTOMYCES GALILAEUS BY A TECHNIQUE OF PROTOPLAST FUSION

Abstract

The technique of protoplast fusion which optimized prototrophic recombination in aclacinomycin-producing Streptomyces galilaeus was studied and applied to the construction of new anthracycline analog-producing recombinant upon genetic cross of two specific mutants blocked in aclacinomycin biosynthesis. Thus, 2-hydroxyaclacinomycin-producing recombinant was obtained by the protoplast fusion.