The Metabolism of Streptomyces Griseus. II The Respiration of Mycelium of Streptomyces Griseus Free from Extraneous Matter

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In the preceding communication⁽¹⁾ of this series, some results of study on the respiration of S. griseus grown on soybean medium with special reference to its relation with the growth age, were reported. But, the data on respiration value mentioned there was considered to be deficient in accuracy, because cell suspensions then used contained some insoluble matters of the media and the accurate measurement of the cell amount was therefore impossible. It was made clear, however, that there existed some differences between young (24 hours) and old (48 hous) cells, namely, that they differ in the sensitivity to streptomycin (SM) and in the oxygen uptake.

In the present study, the cell and the insoluble matter were separated and accurate deta on the oxygen uptake and carbon dioxide output based on experiments with the cells free from extraneous matter will be reported.