Experimental Treatment of Anti-Cancer Substances in the Embryonated Egg

抄録

Mice or rats, inoculated with either ascites or solid tumors have been generally used as tools of experiments for screening of anti-cancer substance. The effect was evaluated on the basis of the growth inhibitory activity against animal tumors and the survived period of host animals. In this paper, embryonated eggs were used as another tool for screening of anti-cancer substance. The cells of Ehrlich carcinoma or Yoshida sarcoma in ascitic form were implanted on the chorioallantoic membrane of the embryonated eggs. After additional incubation for a fewdays, the test solutions were dropped on the choriallantoic membrane or injected into yolk sac. Following incubation for another few days, the eggs were examined. The tumor growth on the chorioallantoic membrane was examined macroscopically and microscopically to evaluate the activity of test samples. The present report deals with the result of the experiments by this method, along with the influence of the anti-tumor substances on the growth of chick-embryo.