Estimation on the Concentration of Nuclear and Tissue Constituents of Rabbit Liver
1959 Volume 16 Issue 1 Pages 81-87
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1959 Volume 16 Issue 1 Pages 81-87
In order to compare the concentration grade of nuclear constituents with that of tissue, four values listed bellow were obtained experimentally. Designs of this work were summarized in Table 1.
1. Ratio of weight of dry matter to its raw tissue (m1/M1 listed in Table 2). The values were estimated by weighing of raw tissue (M1) and of its dry matter after vacuum drying (m1).
2. Specific gravity of liver tissue (M2/V2 listed in Table 3). The values were obtained from weight (M2) and volume (V2) of liver tissue.
3. Mean weight of dry matter of nucleus (Wn listed in Table 4). The values were calculated from whole weight (Wn) and number (N) of freeze-dried nuclei. Procedures for isolation of nuclei were carried out in 0.7% citric acid solution with successive homogenation and centrifugation. Finally obtained nuclear fraction which was suspended in the same madium was frozen and dried completely in vacuo. Whole number of freeze-dried nuclei was calculated from the number counted with hemocytometer under microscope.
4. Mean volume of nucleus (Vn listed in Table 4). The values were calculated from the size in 0.7% citric acid solution observed through Ramsden microocular.
Two ratios: 1./2.=0.328 for tissue, and 3./4.=0.154 for nuclei, may represent the amount of dry matter per its raw volume and may be considered to be “density” of tissue or nucleus respectively. These results may suggest that the “density” of nuclear level may be less than that of tissue, and even if the value was adjusted by HALE and KEY's estimation, it may not exceed the “density” of tissue level.
