Histochemical Studies on the Gland Cells

II. The Chemical Colour Reactions on the Zymogen Granules in the Pancreatic Cells

Abstract

The chemical colour reactions for protein and amino acids were employed on the sections of the rats' pancreas fixed by formalin liquid and embedded in paraffin, and the exocrine pancreatic cells were observed.
The reactions for the indol group (xanthoproteic reaction, COLE aldehyde reaction etc.), for the phenol group (xanthoproteic reaction, PAULY diazo reaction, MILLON reaction etc.) and for the imidazol group (PAULY diazo reaction, tetrazonium reaction and the reaction followed the ‘blocking’ reactions etc.) are definitely positive in zymogen granules. It is noted that the zymogen granules are a peptide made up of, at least, three groups-the indol, the phenol and the imidazol group.
As the result of food administration or the subcutaneous injection of histamine⋅HCl solution, the secretroy activity of the pancreatic cells is initiated and at times an increase, at times a decrease of the zymogen granules can be observed in the cells. But no difference can be seen on the reaction of zymogen granules. There is neither any difference between the zymogen granules in the cell and the granules in the acinus lumen which may be the zymogen granules discharged from the cells.