Microanatomy of the Mouse Osseous Cochlea: A Scanning Electron Microscopic Study

Abstract

This is the first scanning electron microscopic demonstration of the three-dimensional architecture and detailed surface structures of the entire osseous labyrinth of the cochlea. Mouse cochleae were observed after dissolving the soft tissues with KOH and NaOCl solutions.
The precise shapes, surface structures, and orientations of the primary osseous spiral lamina and secondary osseous spiral lamina in the cochlea were observed along their entire course from the hook at the base to the helicotrema at the apex. The primary osseous spiral lamina showed three half turns after the hook; the lengths of the hook and each half turn and the slope angle of the spiral were obtained. The widths of the primary and secondary spiral laminae and the spiral fissure for the basilar membrane between the free edges of the two spiral laminae were measured along the course of the cochlear duct. The surface of the lateral wall under the stria vascularis was also viewed.
Scanning electron microscopy can provide more precise microanatomical data than has been previously available for the osseous cochlea, giving a better understanding of hearing mechanisms with regard to the width, support, and movement of the basilar membrane and the functions of various components of the cochlea.