Abstract
An immunocytochemical study using a monoclonal antibody showed the presence of γ-glutamyl transpeptidase (γ-GTP) in the bile duct epithelium of the normal adult rat liver, while a slight immunoreactivity for γ-GTP could be observed on the membranes of bile canaliculi (the membranes of hepatocytes facing the lumen of bile canaliculi). In hepatocarcinogenesis induced by 3′-methyl-4-dimethylaminoazobenzene, no immunoreactivity was detected in α-fetoprotein-positive cholangiolar “oval” cells, though it was detected on the membranes of bile canaliculi. In livers with hyperplastic nodules, γ-GTP immunoreactivity was observed in clusters, with two different staining patterns. In one pattern, γ-GTP was present not only on the membranes of bile canaliculi, but also on the membranes facing the Disse's space or the lateral cell surface of hepatocytes. In the other pattern, γ-GTP immunoreactivity was seen on the membranes of the dilated bile canaliculi. In livers with developing hepatocellular carcinomas, the immunoreactivity was present on the membranes of irregularly dilated bile canaliculi in malignant cells.
