The Three-dimensional Structure of Tomes' Processes and their Relationship to Arrangement of Enamel Prism in Dog Teeth

Abstract

The relationaship between the course of enamel prisms and the direction of secretory face in Tomes' process of ameloblast was examined three-dimensionally in the dog tooth by light and electron microscopy, as well as by reconstruction.
Scanning electron microscopic observation on the developing enamel surface after the dissolution of the enamel organ showed numerous groups of pits with bulbous faces inclined in the same sidewards direction, while those faces in neighboring groups were inclined in an opposite direction.
Tangential semithin sections from demineralized tooth germs were serially cut from the enamel surface to the enamel-dentin junction. These sections showed numerous belt-like zones arranged perpendicular to the meridian of the tooth. A straight row of enamel prisms perpendicular to the boundaries of the belt-like zones was selected at 142μm from the enamel-dentin junction. The row of prisms and their successive Tomes' processes were reconstructed from micrographs with a personal computer. The cut ends of the enamel prisms initially perpendicular to the boundaries appeared as a sine curve, viewed from the enamel-dentin junction. In a single belt-like zone, the horizontal tilt angles of the enamel prisms towards the enamel-dentin junction tended to be largest at the center of the zone, and smallest near the boundaries. The long axis of each enamel prism was at right angles to the secretory face of the Tomes' process. The secretory faces in a single belt-like zone were inclined in the same sidewards direction and the secretory faces in neighboring zones were inclined in opposite directions.
The results suggest that the direction of the sidewards displacement in ameloblasts is related to that of the secretory face of Tomes' processes. Further, one group of ameloblasts with their secretory faces inclined in the same sidewards direction form one belt-like zone.