Abstract
In this report, present status of a project for a process development based on genomics going on in our groups is explained. Goals of the project supported by the New Energy and Industrial Technology Development Organization (NEDO) is to build object-oriented metabolic pathway models which describe metabolic flux change due to the cultivation conditions change such as from normal one to high osmotic pressure (sugar concentration), and also to apply the model for effectively designing an improved strain producing useful compounds which can play important roles for green chemistry. The model will be developed using the data of transcriptome, proteome and metabolites. In the project, the analysis system which was composed of bioreactor system for taking the on-line/off-line data of metabolic flux analysis, NMR analysis system and software system for data mining from transcriptome as well as proteome, has been set up and took data for high sodium chloride concentration as a model of high osmolarity condition. At the same time, three sub-projects have been proceeded; (1) Basic techniques for metabolic engineering in order to control the targeted pathway flux in Saccharomyces cerevisiae have been developed. (2) Profiling data of transcriptome and proteome have been taken at high sodium chloride concentrations. (3) As one of clustering techniques of the profiling data, SOM (self organizing map) has been extensively studied, applied to the transcriptome data obtained in this project. The project has been now on the stage of strain improvement based on the analysis of the genome information.
