抄録
We created Arg-Gly-Asp (RGD) cell adhesive motif encoding repetitive DNA library with overlap elongation PCR method. By this DNA elongation reaction, we obtained various chain lengths DNAs encoding repetitive RGD, and isolated the genes encoding, 21-times and 43-times repeats of RGD motif. We cloned these genes into the protein expression vector, and overexpressed as thioredoxin fusion proteins, RGD21 and RGD43. The solubility of RGD43 in water is low, and RGD43 formed self-assembled fibrous precipite in water. From the scanning electron microscopic observation, it was indicated that RGD43 formed a branched 3D-network structure in solid state. Mouse fibroblast cells were cultivated on RGD43 protein scaffold, it was indicated that the fibroblast cells adhered and extended long filopodia on RGD43 protein scaffold.
