抄録
In our previous research, a surfactant-lipase complex (lipase complex) that shows high enzymatic activity in organic solvents was immobilized in PEG microsphere using the oil-in-water-in-oil (O/W/O) emulsion. The lipase complex immobilized in PEG microsphere (immobilized lipase complex) exhibited the high enzymatic activity after re-using ten times in organic solvents. In this study, the new method of immobilizing the lipase using the O/W/O emulsion was developed. This new immobilization method do not use the lipase complex. Native lipase is dissolved in PEG buffer solution in the O/W/O multiple emulsion and is immobilized to PEG microsphere during freeze-drying. Native lipase immobilized in PEG microsphere (immobilized native lipase) shows high enzymatic activity that resembled the surfactant-lipase complex and the immobilized surfactant-lipase complex in esterification of lauric acid and benzyl alcohol. Moreover, the high enzymatic activities of the immobilized native lipase were completely maintained also in ten re-using. The performance of the immobilized native lipase showed a heat-resistant property in isooctane. In synthesis of flavor esters, the immobilized native lipase showed the enzymatic activity higher than the lipase complex and the same activity as the immobilized lipase complex. The immobilized native lipase can be a good biocatalyst for the synthesis of flavor esters.
