Abstract
Phylogenetic analyses based on SNPs data detected by MIG-seq was used to reappraise the intraspecific taxonomy of Cassytha filiformis (Lauraceae) in Japan and Taiwan. The network analysis distinguished two terminal clades, one comprising plants with glabrous stems and another comprising plants with pubescent stems. The network analysis also revealed a genetically intermediate clade comprising plants with glabrous and pubescent stems. STRUCTURE analysis revealed the intermediate plants to be of hybrid origin for the first time between the two terminal clades of Cassytha. ML analysis excluding the hybrid plants revealed two clades: one morphologically identifiable as C. filiformis var. filiformis and another identifiable as C. filiformis var. duripraticola based on hairs on the stems following the taxonomic concept of Hatusima (1976).