抄録
The effect of pulsed electric fields (PEF) on the endogenous enzyme of scallops to prevent degradation of ATP-related compounds was investigated. A crude enzyme solution was prepared by dissolving powder of scallop adductor muscle into 0.5 M NaCl solution. The solution was centrifuged at 4000 rpm for 5 min at 4 °C and the resulting supernatant and precipitate were subjected to PEF treatment. After PEF treatment, ATP (final concentration of 1.9 mM) was added as substrate. After reaction time of 0 ~ 24 hours, the enzyme reaction was quenched by adding perchloric acid solutions. ATP-related compounds were determined using liquid chromatography. In the case of the supernatant, the rate of ATP degradation increased with increasing salt concentration and no inactivation of ATP-degrading enzymes was observed by PEF treatment. In the case of precipitates, the ATP degradation rate degradation rate constant was -5.5×10-5 s-1 for the control and -1.2×10-5 s-1 for the PEF sample at 500 mM, indicating a 4.6-fold decrease in reaction rate. This result indicates that the ATP-degrading enzyme was inactivated by PEF treatment.
https://ror.org/04cd75h10 
