Biological Activity of Kidney Cells from the Coho Salmon Immunized with Vibrio anguillarum

Abstract

In order to determine the interaction between chemotactic activity and opsonic effect of, kidney phagocytes from the coho salmon, Oncorhynchus kisutchagainstVibrio anguillarum, fish were immunized once by i. p. injection with formalin killed cells (FKC) ofV. anguillarum.
The interaction between chemotactic activity and opsonic effect was studied in the pooled sera of 8 fishes 1, 3, 5 and 7 weeks after immunization as well as in the kidney phagocytes of immunized and control fish at 12 weeks after immunization.
The agglutinating antibody titer of the pooled sera againstV. anguillarumFKC was determined. The highest chemotactic and phagocytic activity were recorded in the group where pooled immune sera, drawn after 5 weeks of immunization, was mixed with immunized phagocytes.
Moreover, the opsonic effect of the normal serum and its importance has been demonstrated when nonimmune phagocytic cells from the kidney of coho salmon showed an enhanced opsonic activity.
The agglutinating antibody titer began to rise after the 1 st week of immunization with a peak at the 5 th week.