Alizarin Complexone Staining of the Scale and Tissues of Juvenile Red Sea Bream. Pagrus major
1993 Volume 41 Issue 3 Pages 379-385
Details
1993 Volume 41 Issue 3 Pages 379-385
The staining and bleaching processes of the vertebra, gill arch, fin ray, scale, digestive organs, kidney, liver etc by using Alizarin complexone (ALC) were examined in juvenile red sea bream to improve the ALC marking method. Fish in about 15 mm TL were kept in 100 mg⋅L-1 ALC solution for 24 h and then transfered into normal sea water. After rearing in 0, 6, 24, 72, 168 and 264 hrespectively, fish were fixed 70% alcohol. Histological section of juvenile red sea bream were made and observed by using ultraviolet microscope.
Samples just after staining, fluorescent stains were found at many part of both soft and hard tissues. But it was not found at any part, except for scale and otolith, of fish recuperated more than 24h after staining.
Fluorescence were also found in even scale and otolith of fish recuperated 264 hours after staining.
Then we observed and detected fluorescent ring on the scales of recatched fish, they live in four months or more than one year since they were stocked natural waters.
According to past studies, it was concerned that ALC staines was able to detect only at otolith, because that dye was discharged by calcium metabolism. However results of present study certify that staining by ALC is also available to scale.
