Studies on the Immunoglobulin Classes of Incomplete Antiboty in Experimental Hemolytic Anemia Produced in

Abstract

Forty-one rabbits were subjected to the direct antiglobulin technique with specific antiglobulin reagents. Twenty-six rabbits initially proved to be IgG globulin class alone; 12 rabbits initially demonstrated IgG plus IgM globulin class. Very few rabbits started with IgM alone. In most instances, IgG globulin calss autoantibody was constantly observed, while IgM was not always demonstrated during 60 day's observation. In the indirect antiglobulin technique, IgG was initially demonstrated in 10 out of 13 rabbits. IgG was constantly demonstrated on either the direct or the indirect antiglobulin technique, while IgM was not always demonstrated on either antiglobulin technique. Many rabbits initially proved to be IgG type later added by IgM type. IgG globulin class autoantibody seemed to be the main component in anemic rabbits. The antiglobulin technique with specific anti-rabbit c' serum proved to be positive in many instances. But such positive result was not constantly demonstrated in the present study. Therefore it is led to consider that complement component may be very labile. It is considered that incomplete autoantibody against erythrocytes is different from complete antibody against egg-albumin on the basis of the present data that 1) complete antibody against egg-albumin is detected prior to incomplete autoantibody against erythrocytes, 2) initially incomplete autoantibody against erythrocytes proved to be IgG globulin class, but complete antibody against egg-albumin proved to be IgM globulin, and 3) there is no difference in the titer of indirect antiglobulin test of anemic rabbits following absorption with egg-albumin.