Studies on the Immunological Liver Cell Damage : 2. Cytotoxic effects of anti liver antibody upon liver cells

Abstract

Experiments were performed in vitro to study cytotoxic effects of anti liver antibody upon the rat liver cells suspended in Hanks' sol, to which anti rat liver rabbit serum and complement were added, by measuring released enzyme activity and endogenous respiration, and by counting 0.5% trypan blue stained liver cells. The results were as follows: 1. The liver cells, to which anti whole cytoplasm serum and complement were added, showed a high rate of 0.5% trypan blue stained cells and high activity of released enzyme, however, no distinct influence on the endogenous respiration was observed. 2. Antiserum to the mitochondrial fraction, with or without complement, did not revealed any distinct effect upon liver cells. 3. There was a difference in quality between anti whole cytoplasm serum and anti mitochondria serum in respect of the influence on the rat liver cells in vitro.