1970 Volume 19 Issue 3 Pages 193-198,235-23
The fluorimetrical determination of histamine by Shore et al. in 1959, containes many probrems in Japan. This method is not practically suitable for measurment of histamine. Authors have previously been investigated the Shore's method in detail from the point of view as purification of OPT, reaction time and temperature of OPT, the wave's length of fluorescence, the influences of histidine for measurement of histamine etc. Moreover, it is clearly observed the high value in reappearance (means 103%) under our fixed condition. In our method, the determination of histamine in whole blood, shows the value as 0.037-0.075 mcg/ml in normal children. This value was similar in normal adult. The dynamic changes of plasma histamine value may contain many meanings for analysis of biogenic reaction in the body. From these reasons, authors must investigate or discuss above these mentioned problems.