Abstract
Basic studies on some characteristics of ADCC were made using a modified microassay method of cytotoxicity by Takasugi and Klein and culture cells of rat methylcholanthrene sarcoma as target cells. Antisera were obtained by immunizing rabbits or allogeneic rats with the target cells. ADCC activity was much higher with human peripheral blood leucocytes(PBL)than with guinea pig PBL as effector cells and 100 times higher than complement-dependent cytotoxicity(CDC). Lymphoid cells from the spleen and the peripheral blood have much efficient compared with the lymph node cells as ADCC effector cells. Specificity of ADCC with these antisera was clearly demonstrated in this assay system. ADCC activity was dependent on the concentration of the antiserum, the incubation time of cytotoxic assay and the effector dose. Our data also showed that effector cells become cytotoxic after binding of antiserum to target cells but not to effector cells. The existence of Fc receptor on the surface of effector cells was required to the appearance of ADCC.
