1978 Volume 27 Issue 11 Pages 850-855,879-88
In order to analyse surface antigens of human platelets, mixed agglutination with platelets was employed using antiserum to Daudi cells which was known to be absent in β2-microglobulin (β2-m) and HLA. The antiserum reacted with platelet samples taken from 10 individuals with similar antibody titers around 25600. These results could suggest that the antibody(ies) in the serum reacted with antigen(s) commonly distributed on the surface membrane of individual platelets. The absorption of the antiserum was performed with platelets, kidney or spleen cells of various mammalian species. Antibody activity against human platelets were completely removed by absorptions with human spleen cells and platelets, but not with erythrocytes. Chimpanzee platelets and Japanese monkey kidney cells removed also significantly the antibody activity. However, no significant decrease in antibody titers was obtained by absorptions with kidney cells of ox, porcine, rabbit and guinea pig, and also with a mixture of kidney and spleen cells of rat and mouse. The chimpanzee platelets were employed as another target cells and were reacted with rabbit antisera to various human nucleated cells and their membrane components including Daudi cells, platelets, β2-m, and HLA-KCI extract. The obtained results of positive reactions clearly indicated that the chimpanzee platelets contain surface antigens commonly distributed on human platelets and nucleated cells. It was concluded that the major antigen(s) reacting with antiserum to Daudi cells distributed on the surface membranes of both platelets and nucleated cells as the species specific antigens.
