Abstract
A solid phase radioimmunoassay using radiolabeled protein A from staphylococcus aureus has been developed to measure IgG antibody to sugi basic protein (SBP) in sera of sugi sensitive patients. The backgroud counts due to non-specific adsorption of serum IgG on solid phase were minimized by the use of glass rod as solid phase. The values of anti SBP content of 25 human sera obtained with this assay correlated highly (r=0.980, p<0.001) with those obtained with double antibody radioimmunoassay using radiolabeled SBP. This assay could be applied to IgG quantitation for crude allergen extracts as well as purified antigens. The procedure of this assay, using glass rod as solid phase, is as simple as using paper disc as solid phase in RAST. These results indicate that ^<125>I-protein A solid phase radioimmunoassay using glass rod as solid phase may be applicable for measurement of IgG antibodies to many kinds of antigens which are clinically important.
