Bulletin of Glaciological Research
Online ISSN : 1884-8044
Print ISSN : 1345-3807
ISSN-L : 1345-3807
Viabilities of long-term cryopreserved CHO-TRET1 cells with trehalose and DMSO(CHO-TRET1細胞のトレハロースとDMSOによる凍結保存生存率の保存期間依存性)
Tsutomu UCHIDA(内田努)Maho FURUKAWA(古川真帆)Takahiro KIKAWADA(黄川田隆洋)Kenji YAMAZAKI(山崎憲慈)Kazutoshi GOHARA(郷原一寿)
ジャーナル フリー

2019 年 37 巻 p. 1-9


Trehalose acts as a non-toxic cryoprotectant agent (CPA) to CHO-TRET1 cells, which are CHO-K1 cells that express the trehalose transporter. This study examines the viabilities during long-term storage at 193K of cryopreserved CHO-TRET1 cells with trehalose, and compares them to the corresponding viabilities with 10wt%-dimethyl sulfoxide (DMSO). The cryopreserved samples are selected to have a 1-week storage viability exceeding 0.7. Results show that this high viability with trehalose is maintained at the same level for about one year of storage, and gradually decrease to about 0.55±0.16 after about 2.5 years. Comparison of this result with the corresponding case with DMSO suggests that the cryoprotective ability of trehalose is slightly weaker than that of DMSO at this temperature. We discuss the effect of recrystallization of ice, and also the biological damaging, on the viabilities. Then we concluded that the cell membrane slowly degrades at 193K, with the rate being a little faster with trehalose than with DMSO.

© 2019 公益社団法人 日本雪氷学会