Abstract
Ninety strains of Clostridium butyricum/Clostridium beijerinckii group were used in order to find out phenotypic characteristics distinguishing C. butyricum from C. beijerinckii. “C. butyricum” strains can be differentiated from C. beijerinckii strains by the ability to grow in 20% bile, to produce lactic acid from PYG broth and to ferment ribose, and the failure to produce butanol from PYG broth and ferment inositol, melezitose and sorbitol.