Abstract
The objective of this study was to optimize the growth environment for sawdust-based culture of shiitake mushrooms. As conditions during the cultivation stage of production have the largest impact on yield, we focused on this stage and considered the effects of temperature and CO2 concentration on yield. The effects of varying cultivation temperatures between 20°C and 28°C and CO2 concentrations between 1,700 and 4,500 ppm were investigated. Maximum spawning was observed at 22°C, whereas CO2 concentrations did not influence the yield. The previously recommended temperature was 20°C and the recommended CO2 concentration was under 3,000 ppm. As such, it is apparent that air conditioning utilized in the chilling of spawn can be set at a temperature 2°C higher than previously advised and that ventilation can be reduced. These results will reduce energy inputs while lowering production costs.
