Optimization of Polysaccharide Extraction from Medicinal Mushrooms by Combined Enzyme–High Hydrostatic Pressure

Abstract

The polysaccharides of medicinal mushrooms (Agaricus bisporus, Lentinula edodes, Ganoderma lucidum, and Phellinus linteus) extracted by a combination of enzyme, high hydrostatic pressure were investigated with regard to extraction yield, medicinal value, and structural changes. Performing the hot water treatment (95 ℃, 30 min) after enzymatic extraction (cellulose A, pectinase G) under a high hydrostatic pressure (150 MPa or 250 MPa, 45 ℃ for 20 min) resulted in a higher polysaccharide yield and also facilitated extraction of both total sugars and proteins compared to extraction at ambient pressure.