Biocontrol Science
Online ISSN : 1884-0205
Print ISSN : 1342-4815
ISSN-L : 1342-4815
Note
Separation of Viable Histamine-Producing Bacteria from Yellowtail Meat Components by Density Gradient Centrifugation
EMIKO ARAKIKODAI TAKAYAMAMIKAKO SAITOHIDEAKI MATSUOKA
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2009 年 14 巻 1 号 p. 31-34

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Quantitative separation of live cells from food samples is essential for non-culture methods to be validated. In this viewpoint, the feasibility of density gradient centrifugation (DGC) was demonstrated for the first time using samples of yellowtail meat to which Morganella morganii, a histamine producing bacterium had been added. Using a Ficoll density gradient from 50 to 10 w/v % with 10 w/v % steps, meat-free fractions of M. morganii cells were collected in 20-50 w/v % layers. The total cell collection rate ranged from 73-86 % irrespective of the cell density in the range 102-106 cells/200 µl.
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© 2009 The Society for Antibacterial and Antifungal Agents, Japan
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