Separation of Viable Histamine-Producing Bacteria from Yellowtail Meat Components by Density Gradient Centrifugation

EMIKO ARAKI; KODAI TAKAYAMA; MIKAKO SAITO; HIDEAKI MATSUOKA

doi:10.4265/bio.14.31

Note

Separation of Viable Histamine-Producing Bacteria from Yellowtail Meat Components by Density Gradient Centrifugation

EMIKO ARAKI, KODAI TAKAYAMA, MIKAKO SAITO, HIDEAKI MATSUOKA

著者情報

キーワード: Density gradient centrifugation, Viable cell separation, Histamine-producing bacteria, Histamine-producing fish, Morganella morganii

ジャーナルフリー

2009 年 14 巻 1 号 p. 31-34

DOI https://doi.org/10.4265/bio.14.31

詳細

抄録

Quantitative separation of live cells from food samples is essential for non-culture methods to be validated. In this viewpoint, the feasibility of density gradient centrifugation (DGC) was demonstrated for the first time using samples of yellowtail meat to which Morganella morganii, a histamine producing bacterium had been added. Using a Ficoll density gradient from 50 to 10 w/v % with 10 w/v % steps, meat-free fractions of M. morganii cells were collected in 20-50 w/v % layers. The total cell collection rate ranged from 73-86 % irrespective of the cell density in the range 10²-10⁶ cells/200 µl.

責任著者(Corresponding author)

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