Assimilation of Metal Ions Bound to Porphyrins or Porphyrin-Peptides by Vibrio vulnificus, a Human Pathogen Inhabiting Estuarine and Marine Environments

SHIN-ICHI MIYOSHI; TOMOKO SASAKI; NAHOKO KAKU; TAKAHARU INOUE; NATSUKI UOZUMI; YOKO MAEHARA; HIROSHI NAKAO

doi:10.4265/bio.15.1

Original papers

Assimilation of Metal Ions Bound to Porphyrins or Porphyrin-Peptides by Vibrio vulnificus, a Human Pathogen Inhabiting Estuarine and Marine Environments

SHIN-ICHI MIYOSHI ^†, TOMOKO SASAKI ^†, NAHOKO KAKU ^†, TAKAHARU INOUE ^†, NATSUKI UOZUMI ^†, YOKO MAEHARA ^†, HIROSHI NAKAO ^†

著者情報

キーワード: Vibrio vulnificus, Iron source, Heme, Metalloporphyrin.

ジャーナルフリー

2010 年 15 巻 1 号 p. 1-6

DOI https://doi.org/10.4265/bio.15.1

詳細

抄録

Vibrio vulnificus, a ubiquitous microorganism in aquatic environments, causes serious septicemia to the immunocompromised host. In addition to protoheme, this species can utilize Fe-TCPP [ferric tetrakis (4-carboxyphenyl) porphine] as an iron source. In the present study, heme c bound covalently to the protein in cytochrome c, as well as the Fe-TCPP complex formed with a nanopeptide with a high affinity, was found to be useful iron sources for V. vulnificus. This bacterium was also revealed to use Zn-TCPP as a single zinc source. However, other metalloporphyrins such as Mn-TCPP and Pt-TCPP delayed the bacterial growth in the broth containing Fe-TCPP, suggesting interference in the iron assimilation. These results indicate that V. vulnificus may acquire metal ions from both free and peptide-bound metalloporphyrins.

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