Biocontrol Science
Online ISSN : 1884-0205
Print ISSN : 1342-4815
ISSN-L : 1342-4815
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A Collaborative Study on a Method to Detect Salmonella in Food
MICHIKO MIYAHARAMASUMI TAGUCHIMASASHI KANKIAKEMI KAITOMOE ISHIHARAHIROKO KIMATAAKIHIRO GUNJITEIZO TSUKAMOTO
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2010 年 15 巻 2 号 p. 69-73

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Fourteen laboratories with expertise in Salmonella detection in food joined in a collaborative study. The laboratories performed qualitative analyses of ground pork samples using the proposed detection method. Salmonella Typhimurium (hydrogen sulfide-producing strain) and Salmonella Senftenberg (hydrogen sulfide-nonproducing strain) were used for inoculation. Three levels of Salmonella contamination were used for the study (0, 1-10, and 11-100 cfu/25 g). We evaluated the presence of Salmonella in each sample and the serological O group. Unmarked samples delivered to the laboratories were accurately judged to be inoculated or not inoculated with Salmonella at a 99.8% (419/420) detection rate in this collaborative study. The proposed method is suitable as a standard method to detect Salmonella in food.

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© 2010 The Society for Antibacterial and Antifungal Agents, Japan
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