Phenol-Oxidizing Enzyme Expression in Lentinula edodes by the Addition of Sawdust Extract, Aromatic Compounds, or Copper in Liquid Culture Media

抄録

This study examined how the addition of a sawdust extract from Castanopsis cuspidata, several aromatic compounds, and copper affected the expression of a phenol-oxidizing enzyme in the white-rot basidiomycete, Lentinula edodes. Compared to liquid media that had not been supplemented with sawdust extract （MYPG）, MYPG containing low （MYPG-S₁₀₀） or high （MYPG-S₅₀₀） concentrations of sawdust extract had a marked effect on the promotion of mycelial growth. No manganese peroxidase （MnP） production was observed in either MYPG or MYPG-S₁₀₀ media until 35 days after inoculation. However, MnP production was enhanced by culture in MYPG-S₅₀₀, with a marked increase observed suddenly at 14 days after inoculation. Northern blot analysis revealed that the transcription of the lemnp2 gene coding extracellular MnP was initially observed at detectable levels at day 10 after the initial inoculation of MYPG-S₅₀₀, increasing gradually thereafter until days 22-25. However, laccase （Lcc） production was not observed in any of the media until 35 days after inoculation. Addition of 10 mM aromatic compounds - 1,2-benzenediol, 2-methoxyphenol, hydroquinone, and 4-anisidine - into the MYPG-S₅₀₀ medium completely inhibited MnP production and did not enhance any Lcc production. While the addition of 1 or 2 mM Cu²⁺ （CuSO₄・5H₂O） to MYPG-S₅₀₀ medium completely inhibited MnP production, this Cu²⁺ addition caused a marked increase in Lcc production at 17 and 6 days after the addition, respectively.