Roles of Catalase and Trehalose in the Protection from Hydrogen Peroxide Toxicity in Saccharomyces cerevisiae

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The roles of catalase and trehalose in Saccharomyces cerevisiae subject to hydrogen peroxide (H₂O₂) treatment were examined by measuring the catalase activity and intracellular trehalose levels in mutants lacking catalase or trehalose synthetase. Intracellular trehalose was elevated but the survival rate after H₂O₂ treatment remained low in mutants with deletion of the Catalase T gene. On the other hand, deletion of the trehalose synthetase gene increased the catalase activity in mutated yeast to levels higher than those in the wild-type strain, and these mutants exhibited some degree of tolerance to H₂O₂ treatment. These results suggest that Catalase T is critical in the yeast response to oxidative damage caused by H₂O₂ treatment, but trehalose also plays a role in protection against H₂O₂ treatment.