Comparison of Loop-Mediated Isothermal Amplification(LAMP) and Conventional Culture for the Detection of Legionella Species in Hot Spring Water Samples in Japan

KATSUNORI FURUHATA; TOSHIMITSU ANNAKA; MASANARI IKEDO; MASAHUMI FUKUYAMA; SHIN-ICHI YOSHIDA

doi:10.4265/bio.10.117

Comparison of Loop-Mediated Isothermal Amplification(LAMP) and Conventional Culture for the Detection of Legionella Species in Hot Spring Water Samples in Japan

KATSUNORI FURUHATA, TOSHIMITSU ANNAKA, MASANARI IKEDO, MASAHUMI FUKUYAMA, SHIN-ICHI YOSHIDA

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Keywords: Legionella species, Hot spring water, Loop-mediated isothermal amplification (LAMP)

JOURNAL FREE ACCESS

2005 Volume 10 Issue 3 Pages 117-120

DOI https://doi.org/10.4265/bio.10.117

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Published: September 01, 2005 Received: February 01, 2005 Available on J-STAGE: June 28, 2010 Accepted: April 19, 2005 Advance online publication: - Revised: -
Correction information
Date of correction: June 28, 2010 Reason for correction: - Correction: TITLE Details: Wrong : Comparison of Loop-Mediated Isothermal Amplification(LAMP)and Conventional Culture for the Detection ofLegionellaSpecies in Hot Spring Water Samples in Japan
Right : Comparison of Loop-Mediated Isothermal Amplification(LAMP) and Conventional Culture for the Detection of Legionella Species in Hot Spring Water Samples in Japan

Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : Annaka, T., Kojima, T., Ikedo, M., and Furuhata, K.(2004) The detection of Legionellae from environmental water specimens by the LAMP method (in Japanese). Bokin Bobai, 32, 195-201.
Buiding Management Education Center (1999) Guidelines for the control of legionelosis (in Japanese).
Coleman, S. S., Melanson, D. M., Biosca, E. G., and Oliver, J. D.(1996) Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification. App/. Environ. Microbiol., 62, 1378-1382.
Furuhata, K., Okabe, Y., Dogasaki, C., Hara, M., and Fukuyama, M.(2002) Isolation of Legionella spp. from soils in Japan (in Japanese). Bokin Bobai, 30, 555-561.
Horisaka, T., Fujita, K., Iwata, T., Nakadai, A., Okatani, T., Horikita, T., Taniguchi, T., Honda, E., Yokomizo, Y., and Hayashidani, H.(2004) Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification. J. Clin. Microbio/., 42, 5349-5352.
Inoue, H., Noda, A., and Agata, K.(2004a) Detection of Legionella pneumophila with the polymerase chain reaction after treatment with chlorine or hydrogen peroxide (in Japanese). Bokin Bobai, 32, 383-386.
Inoue, H., Noda, A., Agata, K., Annaka, T., Kojima, T., Watari, H., and Ikedo, M.(2004b) Examination of the rapid detection of Legionella from bathwater samples by the loop-mediated isothermal amplification and polymerase chain reaction methods (in Japanese). Bokin Bobai, 32, 481-487.
Masuda, N., Yasukawa, K., Isawa, Y., Horie, R., Saitoh, J., Ishiguro, T., Nakaguchi, Y., Nishibuchi, M., and Hayashi, T.(2004) Rapid detection of tdh and trh mRNAs of Vibrio parahaemolyticus by the transcription-reverse transcription concerted (TRC) method. J. Biosci. Bioeng., 98, 236-243.
Ng, D. L. K., Koh, B. B., Tay, L., and Heng, B. H.(1997) Comparison of polymerase chain reaction and conventional culture for the detection of Legionellae in cooling tower waters in Singapore. Lett. Appl. Microbiol., 24, 214-216.
National Institute of Infectious Diseases, Infectious Diseases Surveillance Center (2003) Legionellosis, April 1999-December 2002, Japan. Infectious Agents Surveillance Rep., 24, 27-28.
Sayan, R., Igarashi, A., Matsuoka, S., and Sakai, M.(2004) Sensitive and rapid detection of Edwardsiellosis in fish by a loop-mediated isothermal amplification. Appl. Environ. Microbiol., 70, 621-624.
Templeton, K. E., Scheltinga, S. A., Graffelman, A. W., van Schie, J. M., Crielaard, J. W., Sillekens, P., van den Broek, P. J., Goossens, H., Beersma, M. F. C., and Claas, E. C. J.(2003) Comparison and evaluation of real-time PCR, real-time nucleic acid sequence-based amplification, conventional PCR, and serology for diagnosis of Mycoplasma pneumoniae. J. Clin. Microbiol., 41, 4366-4371.

Right : Annaka, T., Kojima, T., Ikedo, M., and Furuhata, K.(2004) The detection of Legionellae from environmental water specimens by the LAMP method (in Japanese). Bokin Bobai, 32, 195-201.
Buiding Management Education Center (1999) Guidelines for the control of legionelosis (in Japanese).
Coleman, S. S., Melanson, D. M., Biosca, E. G., and Oliver, J. D.(1996) Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification. Appl. Environ. Microbiol., 62, 1378-1382.
Furuhata, K., Okabe, Y., Dogasaki, C., Hara, M., and Fukuyama, M.(2002) Isolation of Legionella spp. from soils in Japan (in Japanese). Bokin Bobai, 30, 555-561.
Horisaka, T., Fujita, K., Iwata, T., Nakadai, A., Okatani, T., Horikita, T., Taniguchi, T., Honda, E., Yokomizo, Y., and Hayashidani, H.(2004) Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification. J. Clin. Microbio/., 42, 5349-5352.
Inoue, H., Noda, A., and Agata, K.(2004a) Detection of Legionella pneumophila with the polymerase chain reaction after treatment with chlorine or hydrogen peroxide (in Japanese). Bokin Bobai, 32, 383-386.
Inoue, H., Noda, A., Agata, K., Annaka, T., Kojima, T., Watari, H., and Ikedo, M.(2004b) Examination of the rapid detection of Legionella from bathwater samples by the loop-mediated isothermal amplification and polymerase chain reaction methods (in Japanese). Bokin Bobai, 32, 481-487.
Masuda, N., Yasukawa, K., Isawa, Y., Horie, R., Saitoh, J., Ishiguro, T., Nakaguchi, Y., Nishibuchi, M., and Hayashi, T.(2004) Rapid detection of tdh and trh mRNAs of Vibrio parahaemolyticus by the transcription-reverse transcription concerted (TRC) method. J. Biosci. Bioeng., 98, 236-243.
Ng, D. L. K., Koh, B. B., Tay, L., and Heng, B. H.(1997) Comparison of polymerase chain reaction and conventional culture for the detection of Legionellae in cooling tower waters in Singapore. Lett. Appl. Microbiol., 24, 214-216.
National Institute of Infectious Diseases, Infectious Diseases Surveillance Center (2003) Legionellosis, April 1999-December 2002, Japan. Infectious Agents Surveillance Rep., 24, 27-28.
Sayan, R., Igarashi, A., Matsuoka, S., and Sakai, M.(2004) Sensitive and rapid detection of Edwardsiellosis in fish by a loop-mediated isothermal amplification. Appl. Environ. Microbiol., 70, 621-624.
Templeton, K. E., Scheltinga, S. A., Graffelman, A. W., van Schie, J. M., Crielaard, J. W., Sillekens, P., van den Broek, P. J., Goossens, H., Beersma, M. F. C., and Claas, E. C. J.(2003) Comparison and evaluation of real-time PCR, real-time nucleic acid sequence-based amplification, conventional PCR, and serology for diagnosis of Mycoplasma pneumoniae. J. Clin. Microbiol., 41, 4366-4371.

Abstract

We detected Legionella species in 125 samples of hot spring bath water from variousplaces in Japan using the culture and LAMP methods, and compared the results of the 2 methods. Legionella spp. was detected in 40 samples in the culture test, and 38 of these (95.0%) were also positive in the LAMP test, showing a high rate of consistency. Of the 85 negative samples in the culture test, 38 samples (44.7%) were positive in the LAMP test. The positive rate in the LAMP test was higher than that in the current culture test ; the test procedure wassimple, and judgments could be made in a few hours, showing that the LAMP method is useful for the rapid detection of Legionella spp.

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