Isolation of Bacteria which Produce Yeast Cell Wall-Lytic Enzymes and Their Characterization

SEIJI SHIBASAKI; JUN OKADA; YOSHIO NAKAYAMA; TERUHISA YOSHIDA; MITSUYOSHI UEDA

doi:10.4265/bio.13.91

SEIJI SHIBASAKI, JUN OKADA, YOSHIO NAKAYAMA, TERUHISA YOSHIDA, MITSUYOSHI UEDA

著者情報

キーワード: Cellulosimicrobium cellulans, 16S Rdna, Cell wall lysis, β-glucanase

ジャーナルフリー

2008 年 13 巻 3 号 p. 91-96

DOI https://doi.org/10.4265/bio.13.91

詳細

Published: 2008/09/10 Received: 2008/03/08 Available on J-STAGE: 2010/06/28 Accepted: 2008/05/17 Advance online publication: - Revised: -
訂正情報
訂正日: 2010/06/28 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : To isolate bacteria capable of producing enzymes that lyse cell wall, we screened soil samples from Rokko Mo zuntain where is situated in west Japan. The isolated strains KH1, KH2 and KH3 that secrete yeast cell wall lytic enzymes are described herein. The activity of the enzymes, after isolation from culture supernatants, were examined. The lytic enzyme produced by strain KH3 was the most active in both cell wall degradation and β-1, 3 glucanase activity in comparison with commercially available enzymes. This enzyme also had the highest thermo- and pH-stability. On the baisis of partial 16S rDNA sequence analyses, KH1 and KH3 were identified as Cellulosimicrobium sp. and KH2 as Bacillus sp. KH3 was tested by DNADNA hybridization and GC content in an attempt to precisely classify it, because the 16S rDNA sequence similarities to other strains were very high at 99.4%. These chemotaxonomic experiments indicate that KH3 belongs to Cellulosimicrobium cellulans.
Right : To isolate bacteria capable of producing enzymes that lyse cell wall, we screened soil samples from Rokko Mo zuntain where is situated in west Japan. The isolated strains KH1, KH2 and KH3 that secrete yeast cell wall lytic enzymes are described herein. The activity of the enzymes, after isolation from culture supernatants, were examined. The lytic enzyme produced by strain KH3 was the most active in both cell wall degradation and β-1, 3 glucanase activity in comparison with commercially available enzymes. This enzyme also had the highest thermo- and pH-stability. On the baisis of partial 16S rDNA sequence analyses, KH1 and KH3 were identified as Cellulosimicrobium sp. and KH2 as Bacillus sp. KH3 was tested by DNADNA hybridization and GC content in an attempt to precisely classify it, because the 16S rDNA sequence similarities to other strains were very high at 99.4%. These chemotaxonomic experiments indicate that KH3 belongs to Cellulosimicrobium cellulans.

抄録

To isolate bacteria capable of producing enzymes that lyse cell wall, we screened soil samples from Rokko Mo zuntain where is situated in west Japan. The isolated strains KH1, KH2 and KH3 that secrete yeast cell wall lytic enzymes are described herein. The activity of the enzymes, after isolation from culture supernatants, were examined. The lytic enzyme produced by strain KH3 was the most active in both cell wall degradation and β-1, 3 glucanase activity in comparison with commercially available enzymes. This enzyme also had the highest thermo- and pH-stability. On the baisis of partial 16S rDNA sequence analyses, KH1 and KH3 were identified as Cellulosimicrobium sp. and KH2 as Bacillus sp. KH3 was tested by DNADNA hybridization and GC content in an attempt to precisely classify it, because the 16S rDNA sequence similarities to other strains were very high at 99.4%. These chemotaxonomic experiments indicate that KH3 belongs to Cellulosimicrobium cellulans.

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訂正情報

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