Distinctive Effects of Menadione on the In Vivo Catalase Activity and the Adaptation to Hydrogen Peroxide Stress in Yeast Cells

抄録

In order to evaluate the adaptation response to antifungal agents with an action mechanism involving H₂O₂ generation, it is necessary to develop a test fungus properly adapted to H₂O₂. This study reports that the menadione (MD) treatment method is a useful one in the case of 2 yeast strains; Saccharomyces cerevisiae IFO 0565 and Candida albicans ATCC 10231. When these cells were treated with MD at 1mM for 1 h, the enzyme activity of catalase distinctively increased, while the superoxide dismutase activity did not. Thus cells adapted to H₂O₂ showed marked increase in their survival rate against the challenge of H₂O₂ stress.