抄録
The present study was performed to evaluate the efficiency of a method combining immunomagnetic separation (IMS) followed by CIN agar plating and polymerase chain reaction (PCR) for the rapid, specific and sensitive detection of pathogenic Yersinia enterocolitica. The immunomagnetic beads for Y. enterocolitica 03 or 08, prepared in this study, could sensitively and specifically capture serovar 03 or 08. Furthermore, all of pathogenic Y.enterocolitica strains could be detected by PCR with a chromosomal gene (ail) as the target. The proposed method of combining IMS-plating and PCR with ail-primer set was shown to be capable of specifically and sensitively isolating and identifying as little as 10cfu/g of pathogenic Y.enterocolitica in food and human materials within 1-2 days. In addition, the proposed method has the advantage that the bacterial colonies as living cells in the samples are available for investigating the foodborne infections.
