Crystallization of Bowman-Birk Type Protease Inhibitor (Peanut) and Its Complex with Trypsin

抄録

Crystallization and preliminary crystallographic study of Bowman-Birk type protease inhibitors, A-I, A-II, and B-I1I from peanut seeds (Arachis hypogaea), and of the A-II+trypsin complex were carried out. A-II, with 70 amino acid residues, crystallizes in a trigonal system, P3₁21 (or P3₂21), a=71.8, c=65.9Å, Z=12 or 18. The A-I crystal is isomorphous with that of A-II, indicating that the N-terminal residues are in a disordered state in both crystals. The B-III crystal is monoclinic, C2, a=119.6, b=69.6, c=94.2Å, β=115.1°, Z is about 40. The A-Il+trypsin complex crystallizes in an orthorhombic system, P2₁2₁2₁, a=55.5, b=56.0, c=182.1Å, Z=4.