Genetic Control of the Expression of Two Extended Globoglycolipids Carrying Either the Stage Specific Embryonic Antigen-1 or -3 Determinant in Mouse Kidney

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In the preceding paper (J. Biochem. 101, 553-562 (1987)), we reported the structures of two neutral glycolipids (GL-X and GL-Y) purified from mouse kidney, and demonstrated the occurrence of polymorphic variation of these two glycolipids in inbred strains of mice. GL-X was characterized as galactosyljlβ1-3globotetraosylceramide, which was reported to be stage specific embryonic antigen-3 (SSEA-3) by Kannagi et al. (J. Biol. Chem. 258, 8934-8942 (1983)), and GL-Y as 3-fucolactosaminylβ1-6(galactosylβ1-3) globotetraosylceramide, which carries the SSEA-1 eterminant. In order to elucidate the mode of genetic control of GL-X and GL-Y expression, two variants, i.e., BALB/c mice expressing GL-Y and DBA/2 mice lacking GL-Y but expressing GL-X as the major neutral glycolipid, were subjected to mating experiments and glycolipid analysis. F₁ hybrids expressed GL-Y, therefore GL-Y expression is dominant, and DBA/2 mice were determined to be recessive homozygotes. Through analysis of backcross and F₂ mice, DBA/2 mice were demonstrated to carry a single defective autosomal gene, and it is concluded that because of this DBA/2 mice cannot express GL-Y and so accumulate GL-X. An attempt to map the gene controlling GL-Y expression on mouse chromosomes using coat colors and recombinant inbred strains was not successful.