Primary Structures of and Genes for New Ribosomal Proteins A and B in Escherichia coli
1987 年 101 巻 3 号 p. 817-820
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1987 年 101 巻 3 号 p. 817-820
We determined the partial primary structures of and identified the genes for new basic proteins A and B in Escherichia coli ribosomal 50S subunits, found by means of an improved two-dimensional gel electrophoresis method (1, 2). The sequence up to the 17 th amino acid of protein B was in agreement with that of the X gene in the spc operon. The gene for protein A was searched for in the GenBank data base using the sequence up to the 35 th amino acid, and was found at a locus between infC and rplT. The base sequence indicated that protein A contained 64 amino acids and had a molecular weight of 6, 984. We conclude that proteins A and B are intrinsic ribosomal proteins, and propose calling their genes, rpml and rpmJ, respectively.
