Abstract
Biliary bile acids of Alligator mississippiensis were analyzed by gas-liquid chromatography-mass spectrometry after fractionation by silica gel column chromatography. It was shown that the alligator bile contained 12 C27 bile acids and 8 C24 bile acids. In addition to the C27 bile acids, such as 3α, 7α, 12α-trihydroxy-5β-cholestanoic acid, 3α, 7α, 12α-trihydroxy-5α-cholestanoic acid, 3α, 7α-dihydroxy-5β-cholestanoic acid, 3α, 12α-dihydroxy-5β-cholestanoic acid, 7α, 12α-dihydroxy-3-oxo-5β-cholestanoic acid, and 3α, 12α-dihydroxy-7-oxo-5β-cholestanoic acid, identified previously in the bile of A. mississippiensis, 3α, 7β-dihydroxy-5β-cholestanoic acid, 3α, 7β, 12α-trihydroxy-5β-cholestanoic acid, 7β, 12α-dihydroxy-3-oxo-5β-cholestanoic acid, 3α, 7α, 12α, 24-tetrahydroxy-5β-cholestanoic acid, 3α, 7α, 12α, 26-tetrahydroxy-5β-cholestanoic acid, and 1β, 3α, 7α, 12α-tetrahydroxy-5β-cholestanoic acid were newly identified. And in addition to the C24, bile acids, such as chenodeoxycholic acid, ursodeoxycholic acid, cholic acid, and allocholic acid, identified previously, deoxycholic acid, 3α, 7α-dihydroxy-5β-chol-22-enoic acid, 3α, 7α, 12α-trihydroxy-5α-chol-22-enoic acid, and 3α, 7α, 12α-trihydroxy-5β-chol-22-enoic acid were newly identified.
