1987 年 102 巻 5 号 p. 1023-1031
Two major aminopeptidases, an aminopeptidase B and an aminopeptidase M-like enzyme, were purified from human skeletal muscle by DEAE-cellulose, HPLC gel filtration, and hydroxyapatite column chromatographies. The purified aminopep-tidase B exhibits a molecular weight of 76, 000 under both native and denaturing conditions. The activity of the aminopeptidase B is regulated by Cl ions and other anions in vitro. On the other hand, the aminopeptidase M-like enzyme is a mono-meric protein having a molecular weight of 96, 000. It is capable of significantly cleaving Phe-, Leu-, Arg-, and Ala-aminoacyl bonds in the presence of 2-mercapto-ethanol. The pH optima for both enzymes are around 7.0, and bestatin is an effective inhibitor of both enzymes.