A Chicken Embryo-Specific Myosin Light Chain (L₂₃) Appears to Be Identical with One of Brain Myosin Light Chains

抄録

A novel embryo-specific myosin light chain of 23 kDa molecular weight (L₂₃) was found previously in embryonic chicken skeletal, cardiac, and smooth muscles (Takano-Ohmuro et al. (1985) J. Cell Biol. 100, 2025-2030). When we examined myosin in embryonic and adult brain by two-dimensional electrophoresis, 23 kDa myosin light chain present in brain (Burridge & Bray (1975) J. Mol. Biol. 99, 1-14) comigrated with L₂₃. Two monoclonal antibodies, EL-64 and MT-185d, were applied to clarify the identity of the brain 23 kDa myosin light chain and the chicken embryonic muscle L₂₃. The two antibodies recognize different antigenic deter-minants in the L₂₃ molecule; the former antibody is specific for L₂₃, whereas the latter recognizes the sequence common to fast skeletal muscle myosin light chains 1 and 3, and also L₂₃. The immunoblots combined with two-dimensional gel electrophoresis showed that both EL-64 and MT-185d can bind to the brain 23 kDa myosin light chain as well as the chicken embryonic muscle L₂₃. These results indicate that chicken brain and chicken embryonic muscles contain a common myosin light chain of 23 kDa molecular weight.