1988 年 104 巻 6 号 p. 939-945
The primary structure of a pyrimidine base-specific ribonuclease from bovine brain wasdetermined. The sequence determinedisKESAAAKFRRQHMDSGSSSSSNPNYCNQMM KRRRMTHGRCKPVNTFVHESLDDVKAVCSQKNITCKNGHPNCYQSKSTMSITDCGSSKYPNCAYKTSQKQKYITVACEGNPYVPVHFDGAVLLPASPVPSLPPPIIRL.Althoughthe sequence homology of this RNase with bovine pancreatic RNase A is 78.2%, it consists of 140 amino acid residues, and it is 16 amino acid residues longer than RNase A at the carboxylterminal. In addition to an N-glycosylated long carbohydrate chain, the bovine brain RNase has two short O-glycosylated carbohydrate chains at the 129th and the 133rd serine residues. The additional C-terminal tail of the bovine brain RNase has a unique composi-tion: 6 proline, 5 hydrophobic amino acids, and two basic amino acids, arginine and histidine.
