Studies on the Sugar Chains of Interferon-γfrom Human Peripheral-Blood Lymphocytes
1989 年 105 巻 6 号 p. 1034-1039
詳細
1989 年 105 巻 6 号 p. 1034-1039
Sugar chains of interferon-γ(IFN-γ) from human peripheral-blood lymphocytes (PBL) were liberated by hydrazinolysis. After N-acetylation, the reducing end residues of the sugar chains were tagged with 2-aminopyridine and the pyridylamino (PA-) derivatives were purified by gel filtration and reversed-phase HPLC. Five major PA-sugar chains were obtained. The structure of each PA-sugar chain was estimated by comparing its elution times on anion exchange, reversed-phase, and size-fractionation HPLC with those of PA-sugar chains of IFN-γ from the human myelomonocyte cell line HBL-38 (Yamamoto, S. et al. (1989) J. Biochem. 105, 547-555) as standards, and also by comparison of their elution times after partial desialylation. The results showed that IFN-γ(PBL) contained monoand disialo-biantennary structures with 0 or 1mol of fucose residue, as found for IFN-γ(HBL-38), but the N-acetylneuraminy1α2-6 linkage was dominant in IFN-γ(PBL), unlike IFN-γ(HBL-38), which contains both N-acetylneuraminy1α2-3 and α2-6 linkages.
