Nucleotide Sequence of the Gene Encoding NADH Dehydrogenase from an Alkalophile, Bacillus sp. Strain YN-1

抄録

The gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp., was cloned and sequenced. The cloned DNA fragment contained an open reading frame of 1, 557 nucleotides which encodes a polypeptide composed of 519 amino acid residues (M_r 55, 830). The predicted amino acid sequence was consistent with the partial amino acid sequences including the N-terminal and C-terminal sequences determined in a previous study. Sequence comparison with other flavoenzymes revealed high homology between the present dehydrogenase and Escherichia coli thioredoxin reductase.